The theory of DLS can be introduced utilizing a model system of spherical particles in solution. Finally, data analysis of the DLS measurement, and the applications of DLS as well as the comparison against other size-determine techniques will be shown and summarized. Herein a brief introduction of basic theories of DLS will be demonstrated, followed by descriptions and guidance on the instrument itself and the sample preparation and measurement process. Since the scattering pattern of the light is highly correlated to the size distribution of the analyzed particles, the size-related information of the sample could be then acquired by mathematically processing the spectral characteristics of the scattered light. In the DLS experiment, normally a laser provides the monochromatic incident light, which impinges onto a solution with small particles in Brownian motion.Īnd then through the Rayleigh scattering process, particles whose sizes are sufficiently small compared to the wavelength of the incident light will diffract the incident light in all direction with different wavelengths and intensities as a function of time.
Click here to see more posts about zeta potential/DLS Only 8$ for interpretation of your DLS resultsĭynamic light scattering (DLS), which is also known as photon correlation spectroscopy (PCS) or quasi-elastic light scattering (QLS), is a spectroscopy method used in the fields of chemistry, biochemistry, and physics to determine the size distribution of particles (polymers, proteins, colloids, etc.) in solution or suspension.
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